Exercises I
- Search PubMed for articles on »Mody 4 disease« (query Mody 4).
- Restrict your search to the title of all available articles in PubMed.
- Search PubMed for articles on Mody 4 which are published this year.
- Make a PubMed search for ADH1 and restrict your search to the title and abstract of all articles. Which function is given for that ADH1 gene?
- Find citations to articles about the »ethics of cloning«. Check details tag in the PubMed search interface to see how the terms are mapped (rightmost panel on the pubmed query).
- Find out what happens when you enter the following search terms
- B Crohn
- B Crohn[au]
- Crohn B[au]
- Karry Banks Mullis recieved a Nobel Prize in chemistry in 1993, for his invention of the Polymerase Chain Reaction (PCR). Find the Science-paper where he described the PCR method the first time.
- How many articles can be found in PubMed where Craig Venter is given as the first author? In which important project he was involved?
- Find the orignal paper of the first mammal cloned from differentiated somatic cell (»Dolly, sheep«)?
- Make use of the OMIM database at NCBI.
- What is the difference between the query »"breast cancer"« and »breast cancer«?
- What human genes in OMIM are related to the search term »breast cancer«? Which of those genes are located on chromosome 17?
- Use the limits panel of the OMIM search interface to restrict your search and select only those genes which have a known sequence.
- Retrieve the molecular reason of the »fragile x chromosome«. Browse the OMIM database for »fragile site mental retardation 1 gene; FMR1«. What are the clinical features of this disease? What is the inheritance of this disease? What is the molecular genetic basis?
- Which type of genomic sequences are stored in the taxonomy database at NCBI for Elephas maximus (asiatic elephant). Try the Entrez record nucleotide for this species entry?
Retrieve all available nucleotide sequences of this species in Fasta and GenBank format in taxonomy database at NCBI. Use the Entrez records table to retrieve all genes (upper right corner, section gene).
- How many sequences are available?
- Which part of the elephant genome was sequenced?
- The taxonomy database is usefull to retrieve all available sequence data for an organism?
- How many genes are possible to retrieve via this database for the human (homo sapiens) and rat (rattus norvegicus) species?
- How many genes are available for these two species for the X chromosome?
Please direct questions and comments to Martin Haubrock.